Blair Siegfried

Blair Siegfried

  • Associate Dean for Research and Graduate Education
  • Director of the Pennsylvania Agricultural Experiment Station
0217 Ag Admin Bldg
University Park, PA 16802

Dr. Blair Siegfried assumed the role of Associate Dean for Research and Graduate Education and Director of the Pennsylvania Agricultural Experiment Station, effective October 1, 2021. He received his B.S. in Biology from Lock Haven University and his M.S. from the University of Florida, studying bark beetle chemical ecology. He earned his Ph.D. in Entomology from The Pennsylvania State University where his research focused on metabolism of plant defensive chemicals by insect herbivores and how the relationship affects insecticide susceptibility. He continued his interests in understanding insecticide resistance evolution using the German cockroach as a model system during his post-doctoral training at Cornell University. He joined the faculty at the University of Nebraska in 1990, teaching and conducting research in insecticide and environmental toxicology. In 2008, he was awarded the title of Charles Bessey Professor by the University of Nebraska-Lincoln for scholarship and creative activity. From 2015-2021, Siegfried has served as the head of the Entomology and Nematology Department at the University of Florida. Over the course of his career, Dr. Siegfried’s scholarship in research has resulted in over 200 peer reviewed articles, 14 invited reviews and book chapters, and an additional 18 technical articles and proceedings. His work has also resulted in four published patents with an additional three active patent applications. In 2019, he was selected as Fellow of the Entomological Society of America.

Publications

Does prior exposure to larvicides influence dengue virus susceptibility in Aedes aegypti (Diptera: Culicidae)?
Journal of Medical Entomology, Aldridge, Robert L., Alto, Barry W., Connelly, C. Roxanne, Okech, Bernard, Siegfried, Blair, Eastmond, Bradley H., Alomar, Abdullah A., Linthicum, Kenneth J., 2024

A draft Diabrotica virgifera virgifera genome: insights into control and host plant adaption by a major maize pest insect
BMC Genomics, Coates, Brad S., Walden, Kimberly K.O., Lata, Dimpal, Vellichirammal, Neetha Nanoth, Mitchell, Robert F., Andersson, Martin N., McKay, Rachel, Lorenzen, Marcé D., Grubbs, Nathaniel, Wang, Yu Hui, Han, Jinlong, Xuan, Jing Li, Willadsen, Peter, Wang, Huichun, French, B. Wade, Bansal, Raman, Sedky, Sammy, Souza, Dariane, Bunn, Dakota, Meinke, Lance J., Miller, Nicholas J., Siegfried, Blair D., Sappington, Thomas W., Robertson, Hugh M., 2023

Gene editing of the ABC Transporter/White locus using CRISPR/Cas9-mediated mutagenesis in the Indian Meal Moth
Journal of Insect Physiology, Shirk, Bryce D., Shirk, Paul D., Furlong, Richard B., Scully, Erin D., Wu, Ke, Siegfried, Blair D., 2023

RNAi-induced knockdown of white gene in the southern green stink bug (Nezara viridula L.)
Scientific Reports, Souza, Dariane, Christensen, Shawn A., Wu, Ke, Buss, Lyle, Kleckner, Kaylin, Darrisaw, Constance, Shirk, Paul D., Siegfried, Blair D., 2022

LETHAL AND SUBLETHAL CONCENTRATIONS OF FORMULATED LARVICIDES AGAINST SUSCEPTIBLE AEDES AEGYPTI
Journal of the American Mosquito Control Association, Aldridge, Robert L., Alto, Barry W., Connelly, C. Roxanne, Okech, Bernard, Siegfried, Blair, Linthicum, Kenneth J., 2022

Up-regulation of apoptotic- and cell survival-related gene pathways following exposures of western corn rootworm to B. thuringiensis crystalline pesticidal proteins in transgenic maize roots
BMC Genomics, Coates, Brad S., Deleury, Emeline, Gassmann, Aaron J., Hibbard, Bruce E., Meinke, Lance J., Miller, Nicholas J., Petzold-Maxwell, Jennifer, French, B. Wade, Sappington, Thomas W., Siegfried, Blair D., Guillemaud, Thomas, 2021

Influence of Pyrethroid Resistance on Vector Competency for Zika Virus by Aedes aegypti (Diptera: Culicidae)
Journal of Medical Entomology, Parker-Crockett, Casey, Connelly, C. Roxanne, Siegfried, Blair, Alto, Barry, 2021

Molecular characterization of western corn rootworm pyrethroid resistance
Pest Management Science, Souza, Dariane, Siegfried, Blair D., Meinke, Lance J., Miller, Nicholas J., 2021

Contrasting susceptibility of lepidopteran pests to diamide and pyrethroid insecticides in a region of overwintering and migratory intersection
Pest Management Science, Rabelo, Marcelo M., Paula-Moraes, Silvana V., Pereira, Eliseu José G., Siegfried, Blair D., 2020

Dietary RNAi toxicity assay exhibits differential responses to ingested dsRNAs among lady beetles
Pest Management Science, Pan, Huipeng, Yang, Xiaowei, Romeis, Jörg, Siegfried, Blair D., Zhou, Xuguo, 2020

Demographic performance of helicoverpa zea populations on dual and triple-gene Bt cotton
Toxins, Rabelo, Marcelo M., Paula-Moraes, Silvana V., Pereira, Eliseu Jose G., Siegfried, Blair D., 2020

Control of western corn rootworm via RNAi traits in maize: lethal and sublethal effects of Sec23 dsRNA
Pest Management Science, Vélez, Ana M., Fishilevich, Elane, Rangasamy, Murugesan, Khajuria, Chitvan, McCaskill, David G., Pereira, Adriano E., Gandra, Premchand, Frey, Meghan L.F., Worden, Sarah E., Whitlock, Shannon L., Lo, Wendy, Schnelle, Karl D., Lutz, Jamie R., Narva, Kenneth E., Siegfried, Blair D., 2020

Delivery of gene-specific dsRNA by microinjection and feeding induces RNAi response in Sri Lanka weevil, Myllocerus undecimpustulatus undatus Marshall
Pest Management Science, Pinheiro, Daniele H., Taylor, Caitlin E., Wu, Ke, Siegfried, Blair D., 2020

Transcriptional responses of soybean aphids to sublethal insecticide exposure
Insect Biochemistry and Molecular Biology, Enders, Laramy S., Rault, Leslie C., Heng-Moss, Tiffany M., Siegfried, Blair D., Miller, Nicholas J., 2020

Enhanced metabolism and selection of pyrethroid-resistant western corn rootworms (Diabrotica virgifera virgifera LeConte)
Pesticide Biochemistry and Physiology, Souza, Dariane, Jiménez, Arnubio V., Sarath, Gautam, Meinke, Lance J., Miller, Nicholas J., Siegfried, Blair D., 2020

Evaluation of reference genes for real-time quantitative PCR analysis in southern corn rootworm, Diabrotica undecimpunctata howardi (Barber)
Scientific Reports, Basu, Saumik, Pereira, Adriano E., Pinheiro, Daniele H., Wang, Haichuan, Valencia-Jiménez, Arnubio, Siegfried, Blair D., Louis, Joe, Zhou, Xuguo Joe, Vélez, Ana Maria, 2019

Responses of two ladybird beetle species (Coleoptera: Coccinellidae) to dietary RNAi
Pest Management Science, Haller, Simone, Widmer, Franco, Siegfried, Blair D., Zhuo, Xuguo, Romeis, Jörg, 2019

Lethal RNA interference response in the pepper weevil
Journal of Applied Entomology, Wu, Ke, Taylor, Caitlin E., Pinheiro, Daniele H., Skelley, Lucile H., McAuslane, Heather J., Siegfried, Blair D., 2019

Residues of thiamethoxam and mefenoxam in vegetative and floral tissue of soybean at the early reproductive stage resulting from seed treatments
Crop Protection, Camargo, Carolina, Snow, Daniel D., Onanong, Sathaporn, Hunt, Thomas E., Siegfried, Blair D., 2019

Investigation of Cry3Bb1 resistance and intoxication in western corn rootworm by RNA sequencing
Journal of Applied Entomology, Rault, L. C., Siegfried, B. D., Gassmann, A. J., Wang, H., Brewer, G. J., Miller, N. J., 2018

CRISPR/Cas9 mediated knockout of the abdominal-A homeotic gene in fall armyworm moth (Spodoptera frugiperda)
PLoS One, Wu, Ke, Shirk, Paul D., Taylor, Caitlin E., Furlong, Richard B., Shirk, Bryce D., Pinheiro, Daniele H., Siegfried, Blair D., 2018

Rapid and persistent RNAi response in western corn rootworm adults
Pesticide Biochemistry and Physiology, Wu, Ke, Taylor, Caitlin E., Fishilevich, Elane, Narva, Kenneth E., Siegfried, Blair D., 2018

Clathrin-dependent endocytosis is associated with RNAi response in the western corn rootworm, Diabrotica virgifera virgifera LeConte
PLoS One, Pinheiro, Daniele H., Vélez, Ana M., Fishilevich, Elane, Wang, Haichuan, Carneiro, Newton P., Valencia-Jiménez, Arnubio, Valicente, Fernando H., Narva, Kenneth E., Siegfried, Blair D., 2018

microRNA profiling between Bacillus thuringiensis Cry1Ab-susceptible and -resistant European corn borer, Ostrinia nubilalis (Hübner)
Insect Molecular Biology, Yu, T., Li, X., Coates, B. S., Zhang, Q., Siegfried, B. D., Zhou, X., 2018

Knockdown of RNA interference pathway genes in western corn rootworm, Diabrotica virgifera virgifera, identifies no fitness costs associated with Argonaute 2 or Dicer-2
Pesticide Biochemistry and Physiology, Camargo, Carolina, Wu, Ke, Fishilevich, Elane, Narva, Kenneth E., Siegfried, Blair D., 2018

Systemic RNAi in western corn rootworm, Diabrotica virgifera virgifera, does not involve transitive pathways
Insect Science, Li, Huarong, Bowling, Andrew J., Gandra, Premchand, Rangasamy, Murugesan, Pence, Heather E., McEwan, Robert E., Khajuria, Chitvan, Siegfried, Blair D., Narva, Kenneth E., 2018

Distinct fitness costs associated with the knockdown of RNAi pathway genes in western corn rootworm adults
PLoS One, Wu, Ke, Camargo, Carolina, Fishilevich, Elane, Narva, Kenneth E., Chen, Xiuping, Taylor, Caitlin E., Siegfried, Blair D., 2017

Sublethal Effects of vATPase-A and Snf7 dsRNAs on Biology of Southern Corn Rootworm, Diabrotica undecimpunctata howardi Barber
Journal of Economic Entomology, Pereira, Adriano E., Vélez, Ana M., Meinke, Lance J., Siegfried, Blair D., 2017

Thiamethoxam toxicity and effects on consumption behavior in orius insidiosus (hemiptera: Anthocoridae) on soybean
Environmental Entomology, Camargo, Carolina, Hunt, Thomas E., Giesler, Loren J., Siegfried, Blair D., 2017

Influence of calcareous soil on Cry3Bb1 expression and efficacy in the field
Transgenic Research, Wangila, David S., Valencia J, Arnubio, Wang, Haichuan, Siegfried, Blair D., Meinke, Lance J., 2017

Cross-resistance and synergism bioassays suggest multiple mechanisms of pyrethroid resistance in western corn rootworm populations
PLoS One, Pereira, Adriano E., Souza, Dariane, Zukoff, Sarah N., Meinke, Lance J., Siegfried, Blair D., 2017

Patterns of gene expression in Western corn rootworm (Diabrotica virgifera virgifera) neonates, challenged with Cry34ab1, Cry35Ab1 and Cry34/35Ab1, based on next‐generation sequencing
Toxins, Wang, Haichuan, Eyun, Seong‐Il, Arora, Kanika, Tan, Sek Yee, Gandra, Premchand, Moriyama, Etsuko, Khajuria, Chitvan, Jurzenski, Jessica, Li, Huarong, Donahue, Maia, Narva, Ken, Siegfried, Blair, 2017

Dietary risk assessment of v-ATPase A dsRNAs on monarch butterfly larvae
Frontiers in Plant Science, Pan, Huipeng, Yang, Xiaowei, Bidne, Keith, Hellmich, Richard L., Siegfried, Blair D., Zhou, Xuguo, 2017

Fitness costs associated with Cry1F resistance in the European corn borer
Journal of Applied Entomology, Petzold-Maxwell, J. L., Siegfried, B. D., Hellmich, R. L., Abel, C. A., Coates, B. S., Spencer, T. A., Horikoshi, R. J., Gassmann, A. J., 2017

Method Development for Monitoring Bean Leaf Beetle, Cerotoma trifurcata (Forster) (Coleoptera: Chrysomelidae), Susceptibility to Thiamethoxam Seed Treatments on Soybeans
Journal of Agricultural and Urban Entomology, Tietjen, Chelsea L., Hunt, Thomas E., Snow, Daniel D., Cassada, David A., Siegfried, Blair D., 2017

Parameters for successful parental RNAi as an insect pest management tool in Western Corn Rootworm, Diabrotica virgifera virgifera
Genes, Vélez, Ana M., Fishilevich, Elane, Matz, Natalie, Storer, Nicholas P., Narva, Kenneth E., Siegfried, Blair D., 2017

Susceptibility of Spodoptera frugiperda (Lepidoptera: Noctuidae) Field Populations to the Cry1F Bacillus thuringiensis Insecticidal Protein
Florida Entomologist, Silva, Karen F.Da, Spencer, Terence A., Crespo, Andre L.B., Siegfried, Blair D., 2016

RNAi as a management tool for the western corn rootworm, Diabrotica virgifera virgifera
Pest Management Science, Fishilevich, Elane, Vélez, Ana M., Storer, Nicholas P., Li, Huarong, Bowling, Andrew J., Rangasamy, Murugesan, Worden, Sarah E., Narva, Kenneth E., Siegfried, Blair D., 2016

RNAi induced knockdown of a cadherin-like protein (EF531715) does not affect toxicity of Cry34/35Ab1 or Cry3Aa to Diabrotica virgifera virgifera larvae (Coleoptera: Chrysomelidae)
Insect Biochemistry and Molecular Biology, Tan, Sek Yee, Rangasamy, Murugesan, Wang, Haichuan, Vélez, Ana María, Hasler, James, McCaskill, David, Xu, Tao, Chen, Hong, Jurzenski, Jessica, Kelker, Matthew, Xu, Xiaoping, Narva, Kenneth, Siegfried, Blair D., 2016

Assessment of potential risks of dietary rnai to a soil micro-arthropod, sinella curviseta brook (Collembola: Entomobryidae)
Frontiers in Plant Science, Pan, Huipeng, Xu, Linghua, Noland, Jeffrey E., Li, Hu, Siegfried, Blair D., Zhou, Xuguo, 2016

Editorial overview: Pests and resistance: Resistance to Bt toxins in transgenic crops
Current Opinion in Insect Science, Siegfried, Blair, Jurat-Fuentes, Juan Luis, 2016

Cry1F resistance among lepidopteran pests: A model for improved resistance management?
Current Opinion in Insect Science, Vélez, Ana M., Vellichirammal, Neetha Nanoth, Jurat-Fuentes, Juan Luis, Siegfried, Blair D., 2016

Uptake and translocation of imidacloprid, clothianidin and flupyradifurone in seed-treated soybeans
Pest Management Science, Stamm, Mitchell D., Heng-Moss, Tiffany M., Baxendale, Frederick P., Siegfried, Blair D., Blankenship, Erin E., Nauen, Ralf, 2016

Effects of temporal variation in temperature and density dependence on insect population dynamics
Ecosphere, Haridas, C. V., Meinke, Lance J., Hibbard, Bruce E., Siegfried, Blair D., Tenhumberg, Brigitte, 2016

Use of chromatin remodeling ATPases as RNAi targets for parental control of western corn rootworm (Diabrotica virgifera virgifera) and Neotropical brown stink bug (Euschistus heros)
Insect Biochemistry and Molecular Biology, Fishilevich, Elane, Vélez, Ana M., Khajuria, Chitvan, Frey, Meghan L.F., Hamm, Ronda L., Wang, Haichuan, Schulenberg, Greg A., Bowling, Andrew J., Pence, Heather E., Gandra, Premchand, Arora, Kanika, Storer, Nicholas P., Narva, Kenneth E., Siegfried, Blair D., 2016

Impact of Spodoptera frugiperda neonate pretreatment conditions on Vip3Aa19 insecticidal protein activity and laboratory bioassay variation
Pest Management Science, Da Silva, Karen F., Spencer, Terence A., Camargo Gil, Carolina, Siegfried, Blair D., Walters, Frederick S., 2016

Effect of Cry1F maize on the behavior of susceptible and resistant Spodoptera frugiperda and Ostrinia nubilalis
Entomologia Experimentalis et Applicata, Vélez, Ana María, Alves, Analiza P., Blankenship, Erin E., Siegfried, Blair D., 2016

Pyrosequencing the midgut transcriptome of the banana weevil cosmopolites sordidus (germar) (coleoptera: Curculionidae) reveals multiple protease-like transcripts
PLoS One, Valencia, Arnubio, Wang, Haichuan, Soto, Alberto, Aristizabal, Manuel, Arboleda, Jorge W., Eyun, Seong Il, Noriega, Daniel D., Siegfried, Blair, 2016

Developing an in vivo toxicity assay for RNAi risk assessment in honey bees, Apis mellifera L
Chemosphere, Vélez, Ana María, Jurzenski, Jessica, Matz, Natalie, Zhou, Xuguo, Wang, Haichuan, Ellis, Marion, Siegfried, Blair D., 2016

Quantitative trait locus mapping and functional genomics of an organophosphate resistance trait in the western corn rootworm, Diabrotica virgifera virgifera
Insect Molecular Biology, Coates, B. S., Alves, A. P., Wang, H., Zhou, X., Nowatzki, T., Chen, H., Rangasamy, M., Robertson, H. M., Whitfield, C. W., Walden, K. K., Kachman, S. D., French, B. W., Meinke, L. J., Hawthorne, D., Abel, C. A., Sappington, T. W., Siegfried, B. D., Miller, N. J., 2016

Carbon dioxide receptor genes and their expression profile in Diabrotica virgifera virgifera Molecular Biology
BMC Research Notes, Rodrigues, Thais B., Moriyama, Etsuko N., Wang, Hang, Khajuria, Chitvan, Siegfried, Blair D., 2016

Comparative susceptibility of southern and western corn rootworm adults and larvae to vATPase-A and Snf7 dsRNAs
Journal of RNAi and Gene Silencing, Pereira, Adriano E., Carneiro, Newton P., Siegfried, Blair D., 2016

F2 screening for rare resistance alleles of asian corn borer, ostrinia furnacalis guenee (Lepidoptera: Crambidae) in the Philippines
Journal of the International Society for Southeast Asian Agricultural Sciences, Cayabyab, Bonifacio F., Alcantara, Edwin P., Sumalde, Augusto C., Gonzales, Pablito G., Cuaterno, Wilma R., Siegfried, Blair D., Malenab, Maria Charisma, Belen, Josemari M., Ardez, Karen, 2015

Evidence of field-evolved resistance to bifenthrin in western corn rootworm (Diabrotica virgifera virgifera LeConte) populations in western Nebraska and Kansas
PLoS One, Pereira, Adriano E., Wang, Haichuan, Zukoff, Sarah N., Meinke, Lance J., Wade French, B., Siegfried, Blair D., 2015

Evaluating sub-lethal effects of orchard-applied pyrethroids using video-tracking software to quantify honey bee behaviors
Chemosphere, Ingram, Erin M., Augustin, Julie, Ellis, Marion D., Siegfried, Blair D., 2015

Patterns of differential gene expression in adult rotation-resistant and wild-type western corn rootworm digestive tracts
Evolutionary Applications, Chu, Chia Ching, Zavala, Jorge A., Spencer, Joseph L., Curzi, Matías J., Fields, Christopher J., Drnevich, Jenny, Siegfried, Blair D., Seufferheld, Manfredo J., 2015

Parental RNA interference of genes involved in embryonic development of the western corn rootworm, Diabrotica virgifera virgifera LeConte
Insect Biochemistry and Molecular Biology, Khajuria, Chitvan, Vélez, Ana M., Rangasamy, Murugesan, Wang, Haichuan, Fishilevich, Elane, Frey, Meghan L.F., Carneiro, Newton Portilho, Gandra, Premchand, Narva, Kenneth E., Siegfried, Blair D., 2015

Transcriptional analysis of susceptible and resistant European corn borer strains and their response to Cry1F protoxin
BMC Genomics, Nanoth Vellichirammal, Neetha, Wang, Haichuan, Eyun, Seong Il, Moriyama, Etsuko N., Coates, Brad S., Miller, Nicholas J., Siegfried, Blair D., 2015

Long dsRNA but not siRNA initiates RNAi in western corn rootworm larvae and adults
Journal of Applied Entomology, Li, H., Khajuria, C., Rangasamy, M., Gandra, P., Fitter, M., Geng, C., Woosely, A., Hasler, J., Schulenberg, G., Worden, S., Mcewan, R., Evans, C., Siegfried, B., Narva, K. E., 2015

Selection of reference genes for RT-qPCR analysis in the monarch butterfly, Danaus plexippus (L.), a migrating bio-indicator
PLoS One, Pan, Huipeng, Yang, Xiaowei, Bidne, Keith, Hellmich, Richard L., Siegfried, Blair D., Zhou, Xuguo, 2015

Abiotic and biotic stressors causing equivalent mortality induce highly variable transcriptional responses in the soybean aphid
G3: Genes, Genomes, Genetics, Enders, Laramy S., Bickel, Ryan D., Brisson, Jennifer A., Heng-Moss, Tiffany M., Siegfried, Blair D., Zera, Anthony J., Miller, Nicholas J., 2015

Transcriptional response of soybean to thiamethoxam seed treatment in the presence and absence of drought stress
BMC Genomics, Stamm, Mitchell D., Enders, Laramy S., Donze-Reiner, Teresa J., Baxendale, Frederick P., Siegfried, Blair D., Heng-Moss, Tiffany M., 2014

Corn Rootworm: Small Insect, Big Impact
Natural Sciences Education, Sandall, Leah, Lee, Don, Meinke, Lance, Siegfried, Blair, 2014

Validation of reference housekeeping genes for gene expression studies in Western corn rootworm (Diabrotica virgifera virgifera)
PLoS One, Rodrigues, Thaís Barros, Khajuria, Chitvan, Wang, Haichuan, Matz, Natalie, Cardoso, Danielle Cunha, Valicente, Fernando Hercos, Zhou, Xuguo, Siegfried, Blair, 2014

Horizontal gene transfer and functional diversification of plant cell wall degrading polygalacturonases: Key events in the evolution of herbivory in beetles
Insect Biochemistry and Molecular Biology, Kirsch, Roy, Gramzow, Lydia, Theißen, Günter, Siegfried, Blair D., ffrench-Constant, Richard H., Heckel, David G., Pauchet, Yannick, 2014

Fitness costs of Cry1F resistance in fall armyworm, Spodoptera frugiperda
Journal of Applied Entomology, Vélez, A. M., Spencer, T. A., Alves, A. P., Crespo, A. L.B., Siegfried, B. D., 2014

Estimating the frequency of Cry1F resistance in field populations of the European corn borer (Lepidoptera: Crambidae)
Pest Management Science, Siegfried, Blair D., Rangasamy, Murugesan, Wang, Haichuan, Spencer, Terence, Haridas, Chirakkal V., Tenhumberg, Brigitte, Sumerford, Douglas V., Storer, Nicholas P., 2014

Molecular evolution of glycoside hydrolase genes in the western corn rootworm (Diabrotica virgifera virgifera)
PLoS One, Eyun, Seong Il, Wang, Haichuan, Pauchet, Yannick, Ffrench-Constant, Richard H., Benson, Andrew K., Valencia-Jiménez, Arnubio, Moriyama, Etsuko N., Siegfried, Blair D., 2014

Effect of maize lines on larval fitness costs of Cry1F resistance in the european corn borer (Lepidoptera: Crambidae)
Journal of Economic Entomology, Petzold-Maxwell, Jennifer L., Siegfried, Blair D., Hellmich, Richard L., Abel, Craig A., Coates, Brad S., Spencer, Terrence A., Gassmann, Aaron J., 2014

Soybean aphid (Hemiptera: Aphididae) response to soybean plant defense: Stress levels, tradeoffs, and cross-virulence
Environmental Entomology, Enders, Laramy, Bickel, Ryan, Brisson, Jennifer, Heng-Moss, Tiffany, Siegfried, Blair, Zera, Anthony, Miller, Nick, 2014

Expression and characterization of a recombinant endoglucanase from western corn rootworm, in pichia pastoris
Journal of Insect Science, Jiménez, Arnubio Valencia, Wang, Haichuan, Siegfried, Blair D., 2014